Column: Develosil PAHS-5 150 x 4.6 mm
Mobile phase: 
A)Water
B)Methanol
 
 
 
 
Time
 
0 min
 
3.4 min
 
8.1 min
 
20min
 
 
%B
 
80%
 
80%
 
100%
 
100%
Flow rate: 1.5 mL/min
Temperature: 30 C
Detection:UV@254nm
Sample:1)Naphthalene 2)Acenaphthylene 3)Acenaphthene 4)Fluorene 5)Phenanthrene 6)Anthracene 7)Fluoranthene 8)Pyrene 9)Benzo〔a〕anthracene 10)Chrysene 11)Benzo〔b〕fluoranthene 12)Benzo〔k〕fluoranthene 13)Benzo〔a〕pyrene 14)Dibenzo〔a,h〕anthracene 15)Benzo〔g,h,i〕perylene 16)Indeno〔1,2,3-cd〕pyrene