Compound name : N-3,5-DNB-DL-Leucine

Column Trade Name : aq. TFA pH2 / acetonitrile 60/40

Mobile Phase Composition : CHIRALPAK® IA-3

Chromatographic Mode : Reverse Phase

Column Size : 150 x 4.6 mm

Particle Size : 3 μm

Flow rate (ml/min) : 1

Injection amount (mg) : 0.005

Temperature (°C) : 25

Rt1 & Rt2 (min) : 9.27 / 13.53

k'1 & k'2 : 4.3 / 6.74

Detection : UV 254 nm

Alpha value : 1.57

Resolution : 3.75