Compound name : N-3,5-DNB-DL-Leucine

Column Trade Name : CHIRALPAK® IA-3

Mobile Phase Composition : n-hexane / ethanol / TFA 80/20/0.1

Chromatographic Mode : Normal Phase

Column Size : 150 x 4.6 mm

Particle Size : 3 μm

Flow rate (ml/min) : 1

Injection amount (mg) : 0.0025

Temperature (°C) : 25

Rt1 & Rt2 (min) : 4.94 / 6.17

k'1 & k'2 : 1.9 / 2.62

Detection : UV 230 nm

Alpha value : 1.38

Resolution : 5.1