Compound name : N-3,5-DNB-DL-Leucine

Column Trade Name : CHIRALCEL® OD-H

Mobile Phase Composition : n-hexane / 2-propanol / TFA 80/20/0.1

Chromatographic Mode : Normal Phase

Column Size : 250 x 4.6 mm

Particle Size : 5 μm

Flow rate (ml/min) : 1

Injection amount (mg) : 0.005

Temperature (°C) : 25

Rt1 & Rt2 (min) : 5.35 / 8.01

k'1 & k'2 : 0.78 / 1.67

Detection : UV 254 nm

Alpha value : 2.13

Resolution : 7.51