Compound name : N-3,5-DNB-DL-Leucine

Column Trade Name : CHIRALPAK® QN-AX

Mobile Phase Composition : methanol / acetic acid / NH4OAc 98/2/0.5 (v/v/w)

Chromatographic Mode : Polar Phase

Column Size : 150 x 4.6 mm

Particle Size : 5 μm

Flow rate (ml/min) : 1

Injection amount (mg) : 0.01

Temperature (°C) : 25

Rt1 & Rt2 (min) : 5.89 / 62.49

k'1 & k'2 : 2.17 / 32.60

Detection : UV 254 nm

Alpha value : 15.05

Resolution : 28.47