Compound name : N-3,5-DNB-DL-Leucine

Column Trade Name : CHIRAL-HSA

Mobile Phase Composition : NH4OAc 10mM (+5mM heptafluorobutyric acid) pH7.0 / 2-propanol 85/15

Chromatographic Mode : Reverse Phase

Column Size : 100 x 4.0 mm

Particle Size : 5 μm

Flow rate (ml/min) : 0.9

Injection amount (mg) : 0.0005

Temperature (°C) : 20

Rt1 & Rt2 (min) : 6.96 / 10.28

k'1 & k'2 : 5.46 / 8.54

Detection : UV 225 nm

Alpha value : 1.56

Resolution : 2.08