Compound name : N-3,5-DNB-DL-Leucine

Column Trade Name : CHIRALPAK® IB-3

Mobile Phase Composition : aq. TFA pH2 / acetonitrile 50/50

Chromatographic Mode : Reverse Phase

Column Size : 50 x 4.6 mm

Particle Size : 3 μm

Flow rate (ml/min) : 5

Injection amount (mg) : 0.005

Temperature (°C) : 25

Rt1 & Rt2 (min) : 0.35 / 0.6

k'1 & k'2 : 1.89 / 3.97

Detection : UV 254 nm

Alpha value : 2.1

Resolution : 5.68